一株牦牛源御成门沙门菌对小鼠的致病性

doi:10.11843/j.issn.0366-6964.2015.02.023

Abstract

Abstract:

Salmonella onarimon is a rare serotypes in Salmonella，and its pathogenicity has not been reported.The aim of this experiment was to study the pathogenicity of S.onarimon strain swun3736 isolated from yak in BALB/c mice.Using oral method，we studied its pathogenicity by the median lethal dose (LD₅₀)，the dynamic distribution and histopathological changes in tissues of mice.The results showed that the LD₅₀ was 5 × 10²CFU.When infected with 10⁵CFU per mice，bacteria were first isolated from lungs at 24 h PI，and spleen，liver，kidneys and pancreas at 72 h post infection (PI)，while brain and heart at 96 h PI.In histopathological examination，it appeared the degeneration，necrosis，inflammatory cell infiltration and other serious damage in the lung，spleen，liver，heart，kidney and ileum.While excepting for ileum，other intestinal bowels exhibited no obvious lesions，suggesting that the ileum was the invasion portal of swun3736.These results demonstrated that S.onarimon swun3736 has strongly virulence，which indicated that public hygienics significance was worth concerned.

CLC Number:

S852.612

LEI Zhan-dong，ZHANG Bin，YUE Hua，HUANG Zhi-hong，TANG Cheng. The Study on Pathogenicity of a Yak Salmonella onarimon Isolates in Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(2): 339-343.

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References

［1］BARTHEL M，HAPFELMEIER S，QUINTANILLA-MARTÍNEZ L，et al.Pretreatment of mice with streptomycin provides a Salmonella enterica serovar Typhimurium colitis model that allows analysis of both pathogen and host［J］.Infect Immun，2003，71(5)：2839-2858.
［2］RANIERI M L，SHI C，SWITT A I M，et al.Salmonella serovar prediction：Comparison of typing methods with a new procedure based on sequence characterization［J］.J Clin Microbiol，2013，51(6):1786-1797.
［3］陆承平.兽医微生物学［M］.4版.北京：中国农业出版社，2007.
LU C P.Veterinary microbiology ［M］.4th edition.Beijing：China Agriculture Press，2007.(in Chinese)
［4］KISIDA S.A New Salmonella type (Salmonella onarimon)［J］.Kitasato Arch Expl Med，1940，17：1-7.
［5］TÖREC? K, ERDEM B, ÖNGEN B. Türkiye’de 2011 Y?l? Sonuna Kadar ?zolasyonu Bildirilen Salmonella Serovarlar?[J]. Mikrobiyol Bul, 2013, 47(3): 442-460.
［6］YUE H，ZHU X，ZHANG B，et al.Emergence of the virulence plasmid in Salmonella onarimon and Salmonella blegdam from yak［J］.Vet Microbiol，2013，162(1)：296-297.
［7］REED L J，MUENCH H.A simple method of estimating fifty percent endpoints［J］.Am J Epidemiol，1938，27(3)：493-497.
［8］SWEARINGEN M C，PORWOLLIK S，DESAI P T，et al.Virulence of 32 Salmonella strains in mice［J］.PLoS One，2012，7(4)：e36043.
［9］RAGHUNATHAN A，REED J，SHIN S，et al.Constraint-based analysis of metabolic capacity of Salmonella typhimurium during host-pathogen interaction［J］.BMC Syst Biol，2009，3：38.
［10］OHL M E，MILLER S I.Salmonella：a model for bacterial pathogenesis［J］.Annu Rev Med，2001，52：259-274.
［11］HOHMANN A W，SCHMIDT G，ROWLEY D.Intestinal colonization and virulence of Salmonella in mice［J］.Infect Immun，1978，22(3)：763-770.
［12］CARTER P B，COLLINS F M.The route of enteric infection in normal mice［J］.J Exp Med，1974，139(5)：1189-1203.
［13］DAY D W，MANDAL B K，MORSON B C.The rectal biopsy appearances in Salmonella colitis［J］.Histopathology，1978，2(2)：117-131.
［14］CLARK M A，HIRST B H，JEPSON M A.Inoculum composition and Salmonella pathogenicity island 1 regulate M-cell invasion and epithelial destruction by Salmonella typhimurium［J］.Infect Immun，1998，66(2)：724-731.
［15］CLARK M A，JEPSON M A，SIMMONS N L，et al.Preferential interaction of Salmonella typhimurium with mouse Peyer's patch M cells［J］.Res Microbiol，1994，145(7)：543-552.
［16］DENG S X，CHENG A C，WANG M S，et al.Quantitative studies of the regular distribution pattern for Salmonella enteritidis in the internal organs of mice after oral challenge by a specific real-time polymerase chain reaction［J］.World J Gastroenterol，2008，14(5)：782-789.
［17］WATSON K G，HOLDEN D W.Dynamics of growth and dissemination of Salmonella in vivo［J］.Cell Microbiol，2010，12(10)：1389-1397.
［18］SHEPPARD M，WEBB C，HEATH F，et al.Dynamics of bacterial growth and distribution within the liver during Salmonella infection［J］.Cell Microbiol，2003，5(9)：593-600.
［19］CASADO J L，NAVAS E，FRUTOS B，et al.Salmonella lung involvement in patients with HIV infection［J］.Chest，1997，112(5)：1197-1201.
［20］CONLAN J W.Critical roles of neutrophils in host defense against experimental systemic infections of mice by Listeria monocytogenes，Salmonella typhimurium，and Yersinia enterocolitica［J］.Infect Immun，1997，65(2)：630-635.
［21］张斌，朱晓霞，岳华，等.青藏高原部分地区牦牛源沙门菌血清型及毒力基因的调查［J］.畜牧兽医学报，2013，44(7)：1167-1172.
ZHANG B，ZHU X X，YUE H，et al.The survey study on the serovar and virulent gene of yak Salmonella from some areas of Qinghai-Tibetan plateau［J］.Acta Veterinaria et Zootechnica Sinica，2013，44(7)：1167-1172.(in Chinese)
［22］GULIG P A，DANBARA H，GUINEY D G，et al.Molecular analysis of spy virulence genes of the Salmonella virulence plasmids［J］.Mol Microbiol，1993，7(6)：825-830.

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The Study on Pathogenicity of a Yak Salmonella onarimon Isolates in Mice

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